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chick β tubulin iii  (Neuromics)


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    Neuromics chick β tubulin iii
    Figure 2. Human A53T α-synuclein protein transfers from gut cells to adjoining vagal neurons. (A) Intestinal organoids were prepared from an SNCAA53T mouse in which CCK-containing cells express enhanced green fluorescent protein (eGFP), and vagal nodose ganglia neurons were isolated from an Snca–/– mouse lacking endogenous α-synuclein. (B) Representative images of organoids and neurons grown in coculture for 5 days, with eGFP-positive cells (green) in the organoid and <t>β-tubulin</t> <t>III</t> <t>(Tuj1,</t> cyan) highlighting neuronal processes. (C) Representative high-magnification α-synuclein (red) staining of an eGFP-positive EEC. Red arrow indicates localization to a PGP9.5-positive (cyan) process in an Snca–/– mouse neuron. (D and E) Representative images with neuron-specific β-tubulin III (cyan). Surface and (adjacent) intracellular confocal slices are shown. Scale bars are 30 μm for B, 3 μm for C, and 5 μm for D and E.
    Chick β Tubulin Iii, supplied by Neuromics, used in various techniques. Bioz Stars score: 93/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chick+%CE%B2+tubulin+iii/pm38063197-185-26-30?v=Neuromics
    Average 93 stars, based on 83 article reviews
    chick β tubulin iii - by Bioz Stars, 2026-07
    93/100 stars

    Images

    1) Product Images from "Gut mucosal cells transfer α-synuclein to the vagus nerve."

    Article Title: Gut mucosal cells transfer α-synuclein to the vagus nerve.

    Journal: JCI insight

    doi: 10.1172/jci.insight.172192

    Figure 2. Human A53T α-synuclein protein transfers from gut cells to adjoining vagal neurons. (A) Intestinal organoids were prepared from an SNCAA53T mouse in which CCK-containing cells express enhanced green fluorescent protein (eGFP), and vagal nodose ganglia neurons were isolated from an Snca–/– mouse lacking endogenous α-synuclein. (B) Representative images of organoids and neurons grown in coculture for 5 days, with eGFP-positive cells (green) in the organoid and β-tubulin III (Tuj1, cyan) highlighting neuronal processes. (C) Representative high-magnification α-synuclein (red) staining of an eGFP-positive EEC. Red arrow indicates localization to a PGP9.5-positive (cyan) process in an Snca–/– mouse neuron. (D and E) Representative images with neuron-specific β-tubulin III (cyan). Surface and (adjacent) intracellular confocal slices are shown. Scale bars are 30 μm for B, 3 μm for C, and 5 μm for D and E.
    Figure Legend Snippet: Figure 2. Human A53T α-synuclein protein transfers from gut cells to adjoining vagal neurons. (A) Intestinal organoids were prepared from an SNCAA53T mouse in which CCK-containing cells express enhanced green fluorescent protein (eGFP), and vagal nodose ganglia neurons were isolated from an Snca–/– mouse lacking endogenous α-synuclein. (B) Representative images of organoids and neurons grown in coculture for 5 days, with eGFP-positive cells (green) in the organoid and β-tubulin III (Tuj1, cyan) highlighting neuronal processes. (C) Representative high-magnification α-synuclein (red) staining of an eGFP-positive EEC. Red arrow indicates localization to a PGP9.5-positive (cyan) process in an Snca–/– mouse neuron. (D and E) Representative images with neuron-specific β-tubulin III (cyan). Surface and (adjacent) intracellular confocal slices are shown. Scale bars are 30 μm for B, 3 μm for C, and 5 μm for D and E.

    Techniques Used: Isolation, Staining



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    chick β tubulin iii  (Neuromics)
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    Neuromics chick β tubulin iii
    Figure 2. Human A53T α-synuclein protein transfers from gut cells to adjoining vagal neurons. (A) Intestinal organoids were prepared from an SNCAA53T mouse in which CCK-containing cells express enhanced green fluorescent protein (eGFP), and vagal nodose ganglia neurons were isolated from an Snca–/– mouse lacking endogenous α-synuclein. (B) Representative images of organoids and neurons grown in coculture for 5 days, with eGFP-positive cells (green) in the organoid and <t>β-tubulin</t> <t>III</t> <t>(Tuj1,</t> cyan) highlighting neuronal processes. (C) Representative high-magnification α-synuclein (red) staining of an eGFP-positive EEC. Red arrow indicates localization to a PGP9.5-positive (cyan) process in an Snca–/– mouse neuron. (D and E) Representative images with neuron-specific β-tubulin III (cyan). Surface and (adjacent) intracellular confocal slices are shown. Scale bars are 30 μm for B, 3 μm for C, and 5 μm for D and E.
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    Neuromics chick beta tubulin iii
    (A) Intestinal organoids were prepared from a SNCA A53T mouse in which CCK-containing cells express enhanced green fluorescent protein (eGFP), and vagal nodose ganglia neurons were isolated from a Snca −/− mouse lacking endogenous α-synuclein. (B) Representative images of organoids and neurons grown in co-culture for five days, with eGFP-positive cells (green) in the organoid, and <t>β-tubulin</t> (cyan) highlighting neuronal processes. (C) Representative high-magnification α-synuclein (red) staining of an eGFP-positive EEC. Red arrow indicates localization to a PGP9.5 (cyan) positive process in an Snca −/− mouse neuron. (D,E) Representative images with neuron-specific class <t>III</t> tubulin (Tuj1, cyan). Surface and (adjacent) intracellular confocal slices are shown. Scale bars are 30 μm for panel B, 3 μm for panel C, and 5 μm for panels D and E.
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    Image Search Results


    Figure 2. Human A53T α-synuclein protein transfers from gut cells to adjoining vagal neurons. (A) Intestinal organoids were prepared from an SNCAA53T mouse in which CCK-containing cells express enhanced green fluorescent protein (eGFP), and vagal nodose ganglia neurons were isolated from an Snca–/– mouse lacking endogenous α-synuclein. (B) Representative images of organoids and neurons grown in coculture for 5 days, with eGFP-positive cells (green) in the organoid and β-tubulin III (Tuj1, cyan) highlighting neuronal processes. (C) Representative high-magnification α-synuclein (red) staining of an eGFP-positive EEC. Red arrow indicates localization to a PGP9.5-positive (cyan) process in an Snca–/– mouse neuron. (D and E) Representative images with neuron-specific β-tubulin III (cyan). Surface and (adjacent) intracellular confocal slices are shown. Scale bars are 30 μm for B, 3 μm for C, and 5 μm for D and E.

    Journal: JCI insight

    Article Title: Gut mucosal cells transfer α-synuclein to the vagus nerve.

    doi: 10.1172/jci.insight.172192

    Figure Lengend Snippet: Figure 2. Human A53T α-synuclein protein transfers from gut cells to adjoining vagal neurons. (A) Intestinal organoids were prepared from an SNCAA53T mouse in which CCK-containing cells express enhanced green fluorescent protein (eGFP), and vagal nodose ganglia neurons were isolated from an Snca–/– mouse lacking endogenous α-synuclein. (B) Representative images of organoids and neurons grown in coculture for 5 days, with eGFP-positive cells (green) in the organoid and β-tubulin III (Tuj1, cyan) highlighting neuronal processes. (C) Representative high-magnification α-synuclein (red) staining of an eGFP-positive EEC. Red arrow indicates localization to a PGP9.5-positive (cyan) process in an Snca–/– mouse neuron. (D and E) Representative images with neuron-specific β-tubulin III (cyan). Surface and (adjacent) intracellular confocal slices are shown. Scale bars are 30 μm for B, 3 μm for C, and 5 μm for D and E.

    Article Snippet: Primary antibodies used for immunostaining included rabbit CCK (73), rabbit α-synuclein (Abcam catalog ab138501, RRID:AB_2537217, at 1:1,000), guinea pig PGP9.5 (Abcam catalog ab10410, RRID:AB_297150, at 1:100), chick β-tubulin III (Tuj1; Neuromics catalog CH23005, RRID:AB_2210684, at 1:100), and chick GFP (Abcam catalog ab13970, RRID:AB_300798, at 1:1,000).

    Techniques: Isolation, Staining

    (A) Intestinal organoids were prepared from a SNCA A53T mouse in which CCK-containing cells express enhanced green fluorescent protein (eGFP), and vagal nodose ganglia neurons were isolated from a Snca −/− mouse lacking endogenous α-synuclein. (B) Representative images of organoids and neurons grown in co-culture for five days, with eGFP-positive cells (green) in the organoid, and β-tubulin (cyan) highlighting neuronal processes. (C) Representative high-magnification α-synuclein (red) staining of an eGFP-positive EEC. Red arrow indicates localization to a PGP9.5 (cyan) positive process in an Snca −/− mouse neuron. (D,E) Representative images with neuron-specific class III tubulin (Tuj1, cyan). Surface and (adjacent) intracellular confocal slices are shown. Scale bars are 30 μm for panel B, 3 μm for panel C, and 5 μm for panels D and E.

    Journal: bioRxiv

    Article Title: Gut mucosal cells transfer α-synuclein to the vagus nerve

    doi: 10.1101/2023.08.14.553305

    Figure Lengend Snippet: (A) Intestinal organoids were prepared from a SNCA A53T mouse in which CCK-containing cells express enhanced green fluorescent protein (eGFP), and vagal nodose ganglia neurons were isolated from a Snca −/− mouse lacking endogenous α-synuclein. (B) Representative images of organoids and neurons grown in co-culture for five days, with eGFP-positive cells (green) in the organoid, and β-tubulin (cyan) highlighting neuronal processes. (C) Representative high-magnification α-synuclein (red) staining of an eGFP-positive EEC. Red arrow indicates localization to a PGP9.5 (cyan) positive process in an Snca −/− mouse neuron. (D,E) Representative images with neuron-specific class III tubulin (Tuj1, cyan). Surface and (adjacent) intracellular confocal slices are shown. Scale bars are 30 μm for panel B, 3 μm for panel C, and 5 μm for panels D and E.

    Article Snippet: Primary antibodies used for immunostaining included: Rabbit CCK , rabbit α-synuclein (Abcam Cat# ab138501, RRID:AB_2537217, at 1:1000), guinea pig PGP9.5 (Abcam Cat# ab10410, RRID:AB_297150, at 1:100), chick beta-tubulin III, Tuj1 (Neuromics Cat# CH23005, RRID:AB_2210684, at 1:100), and chick GFP (Abcam Cat# ab13970, RRID:AB_300798, at 1:1000).

    Techniques: Isolation, Co-Culture Assay, Staining